Jurkat NFAT-Luciferase Reporter Cell Line
Item | Cat# | Price |
Stable Cell Line | SNB-SP-0006 | $19,80 |
Compound Test Services | CT-001 | $1,850 per 384w plate (Up To 16 cpds Dose) |
Product Description
The NFAT (Nuclear Factor of Activated T-cells) signaling pathway is a crucial pathway that regulates immune responses, cell differentiation, and development. Its activation hinges on changes in intracellular calcium ion concentration. When a cell is stimulated (e.g., by T-cell receptor activation), calcium levels rise, activating the phosphatase calcineurin. Calcineurin then dephosphorylates NFAT proteins, exposing their nuclear localization signals and triggering their translocation from the cytoplasm into the nucleus. Inside the nucleus, NFAT cooperates with other transcription factors (like AP-1) to bind specific sequences on target genes. This initiates the transcription of genes such as cytokines (e.g., Interleukin-2), ultimately driving the activation and proliferation of immune cells.
Screeningbio’s Jurkat/NFAT/Luciferase reporter gene cell line stable transfected with NFAT response element and luciferase gene. Upon stimulated, NFAT pathway was activated and induce luciferase expression.
Product Specifications
Target Type | Signal Pathway |
Species | Human |
HGNC Symbol | |
Accession Number | |
Parental Line | Jurkat |
Lot# | See Vial |
Storage | Liquid Nitrogen |
Data
Target Background
The NFAT (Nuclear Factor of Activated T-cells) signaling pathway is a crucial pathway that regulates immune responses, cell differentiation, and development. Its activation hinges on changes in intracellular calcium ion concentration. When a cell is stimulated (e.g., by T-cell receptor activation), calcium levels rise, activating the phosphatase calcineurin.
Calcineurin then dephosphorylates NFAT proteins, exposing their nuclear localization signals and triggering their translocation from the cytoplasm into the nucleus. Inside the nucleus, NFAT cooperates with other transcription factors (like AP-1) to bind specific sequences on target genes. This initiates the transcription of genes such as cytokines (e.g., Interleukin-2), ultimately driving the activation and proliferation of immune cells.