HeLa GAS Luciferase Reporter Cell Line

Hela GAS-Luciferase Reporter Cell Line

Item	Cat#	Price
Stable Cell Line	SNB-SP-0008	$19,80
Compound Test Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

The GAS (Gamma-interferon Activation Site) signaling pathway is a key branch of the JAK-STAT pathway that responds to cytokines such as interferon-gamma (IFN-γ). When IFN-γ binds to its cell surface receptor, it activates the associated JAK kinases, which then phosphorylate STAT1 proteins. The phosphorylated STAT1 forms homodimers and translocates into the nucleus, where it specifically recognizes and binds to the conserved GAS sequence in the promoter regions of target genes. This binding initiates the transcription of interferon-stimulated genes (ISGs), thereby regulating processes such as immune responses, inflammatory reactions, and antiviral defense. This pathway serves as a crucial bridge connecting innate and adaptive immunity.

Screeningbio’s HeLa/GAS/Luciferase reporter gene cell line stable transfected with GAS response element and luciferase gene. Upon stimulated, GAS pathway was activated and induce luciferase expression.

Product Specifications

Target Type	Signal Pathway
Species	Human
HGNC Symbol
Accession Number
Parental Line	Hela
Lot#	See Vial
Storage	Liquid Nitrogen

Data

HeLa/GAS/Luciferase Agonist Assay. HeLa/GAS/Luciferase reporter cells were treated with IFNγ. The assay was run based on Promega ONE-GLOTM Luciferase Assay System. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software. — **HeLa/GAS/Luciferase Agonist Assay.** HeLa/GAS/Luciferase reporter cells were treated with IFNγ. The assay was run based on Promega ONE-GLO^TM Luciferase Assay System. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software.

Target Background

The GAS (Gamma-interferon Activation Site) signaling pathway is a key branch of the JAK-STAT pathway that responds to cytokines such as interferon-gamma (IFN-γ). When IFN-γ binds to its cell surface receptor, it activates the associated JAK kinases, which then phosphorylate STAT1 proteins. The phosphorylated STAT1 forms homodimers and translocates into the nucleus, where it specifically recognizes and binds to the conserved GAS sequence in the promoter regions of target genes.

This binding initiates the transcription of interferon-stimulated genes (ISGs), thereby regulating processes such as immune responses, inflammatory reactions, and antiviral defense. This pathway serves as a crucial bridge connecting innate and adaptive immunity.