HEK293 ISRE-Luciferase Reporter Cell Line
Item | Cat# | Price |
Stable Cell Line | SNB-SP-0009 | $19,80 |
Compound Test Services | CT-001 | $1,850 per 384w plate (Up To 16 cpds Dose) |
Product Description
The ISRE signaling pathway is a key mechanism through which cells respond to interferons (IFNs) and establish an antiviral state. When type I interferons (e.g., IFNα/β) bind to cell surface receptors, they activate JAK kinases, leading to the phosphorylation of STAT proteins. These form dimers that translocate into the nucleus and specifically bind to the "Interferon-Sensitive Response Element" (ISRE) in the promoter regions of target genes, initiating the transcription of antiviral and immunoregulatory genes. Based on this principle, the engineered HEK293 ISRE reporter cell line stably integrates a firefly luciferase reporter gene controlled by ISRE sequences, enabling sensitive monitoring of JAK/STAT pathway activity. It has been validated for its response to IFNα stimulation and treatment with JAK inhibitors, and is widely used for antiviral drug screening and research into immune modulation mechanisms.
Screeningbio’s HEK293/ISRE/Luciferase reporter gene cell line stable transfected with ISRE response element and luciferase gene. Upon stimulated, ISRE pathway was activated and induce luciferase expression.
Product Specifications
Target Type | Signal Pathway |
Species | Human |
HGNC Symbol | |
Accession Number | |
Parental Line | HEK293 |
Lot# | See Vial |
Storage | Liquid Nitrogen |
Data
Target Background
The ISRE signaling pathway is a key mechanism through which cells respond to interferons (IFNs) and establish an antiviral state. When type I interferons (e.g., IFNα/β) bind to cell surface receptors, they activate JAK kinases, leading to the phosphorylation of STAT proteins. These form dimers that translocate into the nucleus and specifically bind to the "Interferon-Sensitive Response Element" (ISRE) in the promoter regions of target genes, initiating the transcription of antiviral and immunoregulatory genes. Based on this principle, the engineered HEK293 ISRE reporter cell line stably integrates a firefly luciferase reporter gene controlled by ISRE sequences, enabling sensitive monitoring of JAK/STAT pathway activity. It has been validated for its response to IFNα stimulation and treatment with JAK inhibitors, and is widely used for antiviral drug screening and research into immune modulation mechanisms.