Product Description

The activator protein-1 (AP-1) signaling pathway is a key regulator of gene expression in response to diverse extracellular stimuli, including growth factors, cytokines, and stress signals. AP-1 is a transcription factor complex composed mainly of proteins from the JUN, FOS, ATF, and MAF families, which form homo- or heterodimers. Activation of AP-1 is primarily mediated through the MAPK cascades—ERK, JNK, and p38—which phosphorylate c-Jun and other AP-1 components, enhancing their transcriptional activity. Once activated, AP-1 binds to specific DNA sequences (TRE or CRE elements) to regulate genes involved in cell proliferation, differentiation, apoptosis, and inflammation. Dysregulation of AP-1 signaling contributes to cancer progression, chronic inflammation, and metabolic diseases, making it an important target for therapeutic intervention.

Screeningbio’s HEK293/AP-1/Luciferase reporter gene cell line stable transfected with AP-1 response element and luciferase gene. Upon stimulated, AP-1 pathway was activated and induce luciferase expression.