Product Description

The Luminescent Cell Viability Detection Kit (abbreviated as LCV) is a cell viability assay method developed using the luciferase chemiluminescence system. It determines the number of metabolically active cells by quantitatively measuring the ATP content after cell lysis. The basic principle is illustrated in the figure below: after cell lysis, the luciferin (D‑Luciferin) and the stable mutant luciferase in the kit react with intracellular ATP, enabling quantification of viable cells within a certain cell number range.

The LCV reagent is ready‑to‑use; simply add an equal volume of the reagent to the test wells containing the cells. Shake at room temperature for 2–5 minutes to ensure complete cell lysis and thorough mixing, then allow to stand at room temperature for 10 minutes until the luminescent signal reaches its maximum, at which point the signal can be measured. This product is a “glow‑type” reagent with a signal half‑life of up to 3–5 hours, and exhibits excellent storage stability (retaining >90% activity after 30 days at room temperature or 60 days at 2–8 °C). The kit combines outstanding sensitivity and operational flexibility, making it suitable for high‑throughput cell proliferation and cytotoxicity assays.