Product Description

The V2 receptor (vasopressin V2 receptor) is a Gs protein-coupled receptor encoded by the AVPR2 gene, primarily distributed on the surface of renal collecting duct principal cells and vascular endothelial cells. Its core function, upon activation by arginine vasopressin, involves stimulating adenylyl cyclase via Gs protein to increase intracellular cAMP levels, thereby promoting the insertion of aquaporin-2 into the apical membrane of collecting ducts, enhancing water reabsorption to concentrate urine and maintain body water homeostasis. This receptor is the key molecule mediating antidiuretic effects; its gene mutations cause nephrogenic diabetes insipidus, and it serves as the primary target of clinical drugs (e.g., desmopressin) for treating diabetes insipidus and nocturnal enuresis.

ScreeningBio’s HEK293T/Rat V2 β-arrestin cell line is an ideal tool for studying GPCR/β-arrestin interactions. In this system, the GPCR C-terminus is fused to a smallBiT tag, and the β2-arrestin N-terminus is fused to a largeBiT tag. Upon receptor activation, GPCR/β-arrestin interaction brings the two fragments together to reconstitute an active NanoLuc enzyme, which can be quantified using the NanoBiT substrate. This cell line is designed to evaluate a compound’s ability to activate the β-arrestin signaling pathway.