Product Description

Tachykinin receptors are a family of G protein-coupled receptors (GPCRs) comprising three distinct subtypes: neurokinin 1 (NK1), NK2, and NK3 receptors, each exhibiting unique ligand specificity, tissue distribution, and functional roles. These receptors are activated by structurally related neuropeptides—substance P (SP), neurokinin A (NKA), and neurokinin B (NKB)—which share a conserved C-terminal sequence (-Phe-X-Gly-Leu-Met-NH₂).

ScreeningBio’s HEK293T/Rat NK1R β-arrestin cell line is an ideal tool for studying GPCR/β-arrestin interactions. In this system, the GPCR C-terminus is fused to a smallBiT tag, and the β2-arrestin N-terminus is fused to a largeBiT tag. Upon receptor activation, GPCR/β-arrestin interaction brings the two fragments together to reconstitute an active NanoLuc enzyme, which can be quantified using the NanoBiT substrate. This cell line is designed to evaluate a compound’s ability to activate the β-arrestin signaling pathway.