HEK293T/Rat NK1R β-Arrestin Stable Cell
Item | Cat# | Price |
Stable Cell Line | SNB-A-0218C | Inquiry |
Compound Testing Services | CT-001 | $1,850 per 384w plate (Up To 16 cpds Dose) |
Product Description
Tachykinin receptors are a family of G protein-coupled receptors (GPCRs) comprising three distinct subtypes: neurokinin 1 (NK1), NK2, and NK3 receptors, each exhibiting unique ligand specificity, tissue distribution, and functional roles. These receptors are activated by structurally related neuropeptides—substance P (SP), neurokinin A (NKA), and neurokinin B (NKB)—which share a conserved C-terminal sequence (-Phe-X-Gly-Leu-Met-NH₂).
ScreeningBio’s HEK293T/Rat NK1R β-arrestin cell line is an ideal tool for studying GPCR/β-arrestin interactions. In this system, the GPCR C-terminus is fused to a smallBiT tag, and the β2-arrestin N-terminus is fused to a largeBiT tag. Upon receptor activation, GPCR/β-arrestin interaction brings the two fragments together to reconstitute an active NanoLuc enzyme, which can be quantified using the NanoBiT substrate. This cell line is designed to evaluate a compound’s ability to activate the β-arrestin signaling pathway.
Product Specifications
Target Type | GPCR |
Species | Rat |
HGNC Symbol | NK1R |
Accession Number | NM_012667 (Rn) |
Parental Line | HEK293T |
Lot# | See Vial |
Storage | Liquid Nitrogen |
Data
Target Background
Tachykinin receptors are a family of G protein-coupled receptors (GPCRs) comprising three distinct subtypes: neurokinin 1 (NK1), NK2, and NK3 receptors, each exhibiting unique ligand specificity, tissue distribution, and functional roles.
These receptors are activated by structurally related neuropeptides—substance P (SP), neurokinin A (NKA), and neurokinin B (NKB)—which share a conserved C-terminal sequence (-Phe-X-Gly-Leu-Met-NH₂).