Product Description

KISS1R is a G protein-coupled receptor that specifically binds kisspeptin neuropeptides. It is densely concentrated in key hypothalamic nuclei and also expressed in the placenta, pancreas, and gonads. Its core function is to act as the central “switch” for initiating puberty and maintaining reproductive function.By directly stimulating gonadotropin-releasing hormone neurons, it drives the pulsatile release of GnRH, thereby precisely regulating the entire hypothalamic-pituitary-gonadal axis and governing critical reproductive events such as puberty onset, sex hormone secretion, and ovulation. Inactivation or mutation of this receptor pathway leads to hypogonadotropic hypogonadism, while its abnormal activation is associated with precocious puberty.

ScreeningBio’s HEK293T/Rat Kisspeptin β-Arrestin cell line is an ideal tool for studying GPCR/β-arrestin interactions. In this system, the GPCR C-terminus is fused to a smallBiT tag, and the β2-arrestin N-terminus is fused to a largeBiT tag. Upon receptor activation, GPCR/β-arrestin interaction brings the two fragments together to reconstitute an active NanoLuc enzyme, which can be quantified using the NanoBiT substrate. This cell line is designed to evaluate a compound’s ability to activate the β-arrestin signaling pathway.