Product Description

Chemokine-like receptor 2 (CMKLR2), also known as G-protein coupled receptor 1 (GPR1), is a G protein-coupled receptor homologous to CMKLR1. It is primarily expressed on immune cells, particularly monocytes, macrophages, and neutrophils. Its endogenous ligands include pro-resolving mediators such as Resolvin E1. Its core function is to participate in resolving inflammatory responses by regulating the migration of immune cells to inflammatory sites and promoting the resolution of inflammation, playing a key role in host defense, tissue repair, and suppressing excessive inflammation. Consequently, CMKLR2 is considered a potential therapeutic target for treating inflammatory diseases (such as arthritis and colitis) and promoting tissue regeneration.

ScreeningBio’s HEK293T/Rat Chemerin 2 β-arrestin cell line is an ideal tool for studying GPCR/β-arrestin interactions. In this system, the GPCR C-terminus is fused to a smallBiT tag, and the β2-arrestin N-terminus is fused to a largeBiT tag. Upon receptor activation, GPCR/β-arrestin interaction brings the two fragments together to reconstitute an active NanoLuc enzyme, which can be quantified using the NanoBiT substrate. This cell line is designed to evaluate a compound’s ability to activate the β-arrestin signaling pathway.