Product Description

The neurotensin receptor 1 (NTS1) is a high-affinity G protein-coupled receptor for neurotensin. It is primarily distributed in the central nervous system (e.g., substantia nigra, ventral tegmental area, hypothalamus, and amygdala) and the gastrointestinal tract (e.g., enteroendocrine cells and myenteric neurons). Its core function, mediated via Gq protein signaling, involves regulating dopaminergic neurotransmission, appetite suppression, visceral pain perception, as well as intestinal secretion and motility. It is closely associated with the pathophysiology of conditions such as schizophrenia, addiction, obesity, and certain gastrointestinal cancers.

ScreeningBio’s HEK293T/Mouse NTS1 β-arrestin cell line is an ideal tool for studying GPCR/β-arrestin interactions. In this system, the GPCR C-terminus is fused to a smallBiT tag, and the β2-arrestin N-terminus is fused to a largeBiT tag. Upon receptor activation, GPCR/β-arrestin interaction brings the two fragments together to reconstitute an active NanoLuc enzyme, which can be quantified using the NanoBiT substrate. This cell line is designed to evaluate a compound’s ability to activate the β-arrestin signaling pathway.