Product Description

Neuropeptide FF receptor 2 (NPFF2) is a member of the G protein-coupled receptor family, predominantly distributed in the central nervous system with notable expression in regions associated with emotion, endocrine regulation, and pain modulation, such as the hippocampus, hypothalamus, and spinal dorsal horn. Its core functions involve modulating opioid system activity, influencing pain processing, opioid tolerance, and addiction, as well as participating in blood pressure regulation, stress responses, and energy balance. NPFF2 serves as a key receptor mediating the anti-opioid or modulatory effects of neuropeptide FF on endogenous opioid actions.

ScreeningBio’s HEK293T/Mouse NPFF2 β-arrestin cell line is an ideal tool for studying GPCR/β-arrestin interactions. In this system, the GPCR C-terminus is fused to a smallBiT tag, and the β2-arrestin N-terminus is fused to a largeBiT tag. Upon receptor activation, GPCR/β-arrestin interaction brings the two fragments together to reconstitute an active NanoLuc enzyme, which can be quantified using the NanoBiT substrate. This cell line is designed to evaluate a compound’s ability to activate the β-arrestin signaling pathway.