Product Description

Neuropeptide FF receptor 1 (NPFF1) is a G protein-coupled receptor primarily distributed in the central nervous system, especially in regions associated with pain perception and neuroendocrine regulation such as the spinal cord, brainstem, and hypothalamus. Its main functions include modulating pain sensation, particularly mediating thermal and inflammatory pain, and exerting anti-opioid effects. Additionally, it is involved in regulating cardiovascular activity, feeding, and stress responses, serving as a key receptor for mediating the physiological and pharmacological effects of neuropeptide FF.

ScreeningBio’s HEK293T/Mouse NPFF1 β-arrestin cell line is an ideal tool for studying GPCR/β-arrestin interactions. In this system, the GPCR C-terminus is fused to a smallBiT tag, and the β2-arrestin N-terminus is fused to a largeBiT tag. Upon receptor activation, GPCR/β-arrestin interaction brings the two fragments together to reconstitute an active NanoLuc enzyme, which can be quantified using the NanoBiT substrate. This cell line is designed to evaluate a compound’s ability to activate the β-arrestin signaling pathway.