Product Description

The secretin receptor is a class B G protein-coupled receptor primarily located in the stomach, pancreatic ductal epithelial cells, kidneys, and central nervous system. Its key function is to respond to the intestinal hormone secretin by activating the Gs protein-cAMP signaling pathway, potently stimulating the pancreas to secrete bicarbonate-rich fluid to neutralize duodenal acidity, while also inhibiting gastric acid secretion and modulating renal water-electrolyte balance. This receptor plays a critical role in regulating digestive tract pH and secretory functions, and its dysfunction is associated with diseases such as chronic pancreatitis, peptic ulcers, and gastrinoma.

ScreeningBio’s HEK293T/Human Secretin β-Arrestin cell line is an ideal tool for studying GPCR/β-arrestin interactions. In this system, the GPCR C-terminus is fused to a smallBiT tag, and the β2-arrestin N-terminus is fused to a largeBiT tag. Upon receptor activation, GPCR/β-arrestin interaction brings the two fragments together to reconstitute an active NanoLuc enzyme, which can be quantified using the NanoBiT substrate. This cell line is designed to evaluate a compound’s ability to activate the β-arrestin signaling pathway.