HEK293T Human BB1 β-Arrestin Stable Cell

HEK293T/Human BB1 β-Arrestin Stable Cell

Item	Cat#	Price
Stable Cell Line	SNB-A-0035A	Inquiry
Compound Testing Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

The Neuromedin B receptor (BB1) is a seven-transmembrane G protein-coupled receptor that binds to its endogenous ligand, neuromedin B. This neuropeptide functions as a growth factor and mitogen in gastrointestinal epithelial tissues as well as in normal and neoplastic lung tissues. The receptor is involved in regulating various physiological processes including smooth muscle contraction, neuronal responses, and cellular growth. Research indicates that antagonists of this receptor hold potential therapeutic value in inhibiting tumor cell growth, while genetic polymorphisms of the receptor may be associated with susceptibility to schizophrenia.

ScreeningBio’s HEK293T/Human BB1 β-Arrestin cell line is an ideal tool for studying GPCR/β-arrestin interactions. In this system, the GPCR C-terminus is fused to a smallBiT tag, and the β2-arrestin N-terminus is fused to a largeBiT tag. Upon receptor activation, GPCR/β-arrestin interaction brings the two fragments together to reconstitute an active NanoLuc enzyme, which can be quantified using the NanoBiT substrate. This cell line is designed to evaluate a compound’s ability to activate the β-arrestin signaling pathway.

Product Specifications

Target Type	GPCR
Species	Human
HGNC Symbol	BB1
Accession Number	NM_002511 (Hs)
Parental Line	HEK293T
Lot#	See Vial
Storage	Liquid Nitrogen

Data

HEK293T/Human BB1 β-Arrestin Agonist Assay. HEK293T/Human BB1 β-Arrestin cells were treated with the reference agonist. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 /IC50 values were determined, using GraphPad Prism software. — **HEK293T/Human BB1 β-Arrestin Agonist Assay.** HEK293T/Human BB1 β-Arrestin cells were treated with the reference agonist. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 /IC50 values were determined, using GraphPad Prism software.

Target Background

The Neuromedin B receptor (BB1) is a seven-transmembrane G protein-coupled receptor that binds to its endogenous ligand, neuromedin B. This neuropeptide functions as a growth factor and mitogen in gastrointestinal epithelial tissues as well as in normal and neoplastic lung tissues.

The receptor is involved in regulating various physiological processes including smooth muscle contraction, neuronal responses, and cellular growth. Research indicates that antagonists of this receptor hold potential therapeutic value in inhibiting tumor cell growth, while genetic polymorphisms of the receptor may be associated with susceptibility to schizophrenia.