HEK293 Human PAR1 Stable Cell

HEK293/Human PAR1 Stable Cell

Item	Cat#	Price
Stable Cell Line	SNB-G-0203A	$19,800
Compound Testing Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

PAR1 receptor (Protease-Activated Receptor 1) is a G protein-coupled receptor activated by thrombin, belonging to the protease-activated receptor family. It is widely distributed on platelets, vascular endothelial cells, fibroblasts, smooth muscle cells, and in the central nervous system. Its core function involves a unique self-activation mechanism—thrombin cleaves the receptor's N-terminus to expose a new binding site, thereby triggering intracellular signaling cascades that mediate platelet aggregation, inflammatory responses, vasomotor regulation, and tissue repair. This receptor plays a critical role in cardiovascular diseases such as thrombosis, atherosclerosis, and restenosis, making it an important target for antiplatelet drug development.

Screeningbio’s HEK293/Human PAR1 cell line overexpress F2R receptor and is designed to detect increases in intracellular calcium flux signal in response to agonist stimulation of the receptor. Molecular Devices Calcium 6 kit can be used to detect the signal.

Product Specifications

Target Type	GPCR
Species	Human
HGNC Symbol	F2R
Accession Number	NM_001992 (Hs)
Parental Line	HEK293
Lot#	See Vial
Storage	Liquid Nitrogen

Data

HEK293/Human PAR1 Agonist Assay. HEK293/Human PAR1 cells were treated with the reference agonist. The assay was run based on FLIPR Calcium 6 Assay protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software. — **HEK293/Human PAR1 Agonist Assay.** HEK293/Human PAR1 cells were treated with the reference agonist. The assay was run based on FLIPR Calcium 6 Assay protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software.

Target Background

PAR1 receptor (Protease-Activated Receptor 1) is a G protein-coupled receptor activated by thrombin, belonging to the protease-activated receptor family.

It is widely distributed on platelets, vascular endothelial cells, fibroblasts, smooth muscle cells, and in the central nervous system. Its core function involves a unique self-activation mechanism—thrombin cleaves the receptor's N-terminus to expose a new binding site, thereby triggering intracellular signaling cascades that mediate platelet aggregation, inflammatory responses, vasomotor regulation, and tissue repair.

This receptor plays a critical role in cardiovascular diseases such as thrombosis, atherosclerosis, and restenosis, making it an important target for antiplatelet drug development.