HEK293 Human MT1 Stable Cell

HEK293/Human MT1 Stable Cell

Item	Cat#	Price
Stable Cell Line	SNB-G-0147A	$19,800
Compound Testing Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

MT1 (Melatonin Receptor 1A, MTNR1A) is one of the two primary high-affinity G protein-coupled receptors for melatonin. It is predominantly distributed in the suprachiasmatic nucleus of the brain, retina, pituitary gland, and gonadal tissues. Its core function is to mediate the rhythmic signals of melatonin, acting as a critical "circadian coordinator." By inhibiting cAMP production via Gi/o protein signaling, it regulates circadian rhythms, sleep-wake cycles, and participates in seasonal reproduction, thermoregulation, and immune function modulation. The diurnal fluctuation of its expression and activity forms the molecular foundation linking environmental light-dark cycles to intrinsic physiological functions.

Screeningbio’s HEK293/Human MT1 cell line inducible express MTNR1A and is designed to detect inhibition in intracellular cAMP levels in response to agonist stimulation of the receptor. Cisbio HTRF cAMP kit can be used to detect the signal.

Product Specifications

Target Type	GPCR
Species	Human
HGNC Symbol	MTNR1A
Accession Number	SNB-G-0147A
Parental Line	HEK293
Lot#	See Vial
Storage	Liquid Nitrogen

Data

HEK293/Human MT1 Agonist Assay. HEK293/Human MT1 cells were stimulated with 5 μM Forskolin and treated with reference agonist. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software. — **HEK293/Human MT1 Agonist Assay.** HEK293/Human MT1 cells were stimulated with 5 μM Forskolin and treated with reference agonist. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software.

Target Background

MT1 (Melatonin Receptor 1A, MTNR1A) is one of the two primary high-affinity G protein-coupled receptors for melatonin. It is predominantly distributed in the suprachiasmatic nucleus of the brain, retina, pituitary gland, and gonadal tissues.

Its core function is to mediate the rhythmic signals of melatonin, acting as a critical "circadian coordinator." By inhibiting cAMP production via Gi/o protein signaling, it regulates circadian rhythms, sleep-wake cycles, and participates in seasonal reproduction, thermoregulation, and immune function modulation.

The diurnal fluctuation of its expression and activity forms the molecular foundation linking environmental light-dark cycles to intrinsic physiological functions.