HEK293 Human GnRH Stable Cell

HEK293/Human GnRH Stable Cell

Item	Cat#	Price
Stable Cell Line	SNB-G-0111A	$19,800
Compound Testing Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

Gonadotropin-releasing hormone 1 (GNRH1) is a decapeptide neurohormone synthesized and secreted by hypothalamic neurons. It acts specifically on gonadotrope cells in the anterior pituitary via the hypophyseal portal system. Its core function is to stimulate, in a pulsatile manner, the synthesis and secretion of luteinizing hormone and follicle-stimulating hormone, thereby initiating and regulating the entire hypothalamic-pituitary-gonadal axis. It governs critical reproductive processes such as puberty onset, gametogenesis, and sex hormone secretion. The secretory pattern and function of GNRH1 are directly linked to reproductive health, and its analogs or antagonists are widely used in clinical treatments for assisted reproduction and sex hormone-dependent tumors.

Screeningbio’s HEK293/Human GnRH cell line overexpress GNRHR receptor and is designed to detect increases in intracellular Calcium flux signal in response to agonist stimulation of the receptor. Molecular Devices Calcium 6 kit can be used to detect the signal.

Product Specifications

Target Type	GPCR
Species	Human
HGNC Symbol	GNRHR
Accession Number	NM_000406 (Hs)
Parental Line	HEK293
Lot#	See Vial
Storage	Liquid Nitrogen

Data

HEK293/Human GnRH Agonist Assay. HEK293/Human GnRH cells were treated with the reference agonist. The assay was run based on FLIPR Calcium 6 Assay protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software. — **HEK293/Human GnRH Agonist Assay.** HEK293/Human GnRH cells were treated with the reference agonist. The assay was run based on FLIPR Calcium 6 Assay protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software.

Target Background

Gonadotropin-releasing hormone 1 (GNRH1) is a decapeptide neurohormone synthesized and secreted by hypothalamic neurons. It acts specifically on gonadotrope cells in the anterior pituitary via the hypophyseal portal system.

Its core function is to stimulate, in a pulsatile manner, the synthesis and secretion of luteinizing hormone and follicle-stimulating hormone, thereby initiating and regulating the entire hypothalamic-pituitary-gonadal axis. It governs critical reproductive processes such as puberty onset, gametogenesis, and sex hormone secretion.

The secretory pattern and function of GNRH1 are directly linked to reproductive health, and its analogs or antagonists are widely used in clinical treatments for assisted reproduction and sex hormone-dependent tumors.