HEK293 Human EP4 Stable Cell

HEK293/Human EP4 Stable Cell

Item	Cat#	Price
Stable Cell Line	SNB-G-0199A	$19,800
Compound Testing Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

Ask For A Custom Quote

Product Description

The EP4 receptor (Prostaglandin E receptor 4) is one of the four G protein-coupled receptor subtypes for prostaglandin E2, primarily coupling to the Gs protein. It is widely distributed in the cardiovascular system (vascular endothelium, myocardium), kidneys (glomeruli, collecting ducts), gastrointestinal tract, uterus, lungs, bone, and various immune cells. Its core function involves activating adenylyl cyclase to elevate intracellular cAMP levels, thereby mediating vasodilation, inhibition of immune cell activation, promotion of bone formation, gastric mucosal protection, regulation of renal water and sodium excretion, and modulation of inflammation resolution processes. This receptor plays critical roles in conditions such as ductus arteriosus closure, bone metabolic balance, inflammatory bowel disease, and rheumatoid arthritis, making it an important therapeutic target for pulmonary arterial hypertension and inflammatory disorders.

Screeningbio’s HEK293/Human EP4 cell line overexpress PTGER4 and is designed to detect increases in intracellular cAMP levels in response to agonist stimulation of the receptor. Cisbio HTRF cAMP kit can be used to detect the signal.

Product Specifications

Target Type	GPCR
Species	Human
HGNC Symbol	PTGER4
Accession Number	NM_000958 (Hs)
Parental Line	HEK293
Lot#	See Vial
Storage	Liquid Nitrogen

Data

HEK293/Human EP4 Agonist Assay. HEK293/Human EP4 cells were treated with the reference agonist. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software. — **HEK293/Human EP4 Agonist Assay.** HEK293/Human EP4 cells were treated with the reference agonist. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software.

Target Background

The EP4 receptor (Prostaglandin E receptor 4) is one of the four G protein-coupled receptor subtypes for prostaglandin E2, primarily coupling to the Gs protein.

It is widely distributed in the cardiovascular system (vascular endothelium, myocardium), kidneys (glomeruli, collecting ducts), gastrointestinal tract, uterus, lungs, bone, and various immune cells. Its core function involves activating adenylyl cyclase to elevate intracellular cAMP levels, thereby mediating vasodilation, inhibition of immune cell activation, promotion of bone formation, gastric mucosal protection, regulation of renal water and sodium excretion, and modulation of inflammation resolution processes.

This receptor plays critical roles in conditions such as ductus arteriosus closure, bone metabolic balance, inflammatory bowel disease, and rheumatoid arthritis, making it an important therapeutic target for pulmonary arterial hypertension and inflammatory disorders.