HEK293 Human EP1 Stable Cell

HEK293/Human EP1 Stable Cell

Item	Cat#	Price
Stable Cell Line	SNB-G-0196A	$19,800
Compound Testing Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

The prostaglandin E receptor 1 (EP1) is a Gq protein-coupled receptor subtype for prostaglandin E2 (PGE2), widely distributed in the kidneys, gastrointestinal tract, uterine smooth muscle, blood vessels, and sensory neurons. Its main function is to activate phospholipase C, mediating intracellular calcium mobilization, thereby promoting smooth muscle contraction, enhancing neuropathic pain perception, and regulating renal water and salt reabsorption. This receptor plays a significant role in inflammatory pain, fever, and the progression of certain cancers.

Screeningbio’s HEK293/Human EP1 cell line overexpress PTGER1 receptor and is designed to detect increases in intracellular IP-1 levels in response to agonist stimulation of the receptor. Cisbio HTRF IP-1 kit can be used to detect the signal.

Product Specifications

Target Type	GPCR
Species	Human
HGNC Symbol	PTGER1
Accession Number	NM_000955 (Hs)
Parental Line	HEK293
Lot#	See Vial
Storage	Liquid Nitrogen

Data

HEK293/Human EP1 Agonist Assay. HEK293/Human EP1 cells were treated with the reference agonist. The assay was run based on Revvity IP-one HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 /IC50 values were determined, using GraphPad Prism software. — **HEK293/Human EP1 Agonist Assay.** HEK293/Human EP1 cells were treated with the reference agonist. The assay was run based on Revvity IP-one HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 /IC50 values were determined, using GraphPad Prism software.

Target Background

The prostaglandin E receptor 1 (EP1) is a Gq protein-coupled receptor subtype for prostaglandin E2 (PGE2), widely distributed in the kidneys, gastrointestinal tract, uterine smooth muscle, blood vessels, and sensory neurons.

Its main function is to activate phospholipase C, mediating intracellular calcium mobilization, thereby promoting smooth muscle contraction, enhancing neuropathic pain perception, and regulating renal water and salt reabsorption. This receptor plays a significant role in inflammatory pain, fever, and the progression of certain cancers.