HEK293 Human CXCR2 Stable Cell

HEK293/Human CXCR2 Stable Cell

Item	Cat#	Price
Stable Cell Line	SNB-G-0063A	$19,800
Compound Testing Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

C-X-C motif chemokine receptor 2 (CXCR2) is a G protein-coupled receptor that primarily binds CXC family chemokines (such as IL-8/CXCL8 and GROα/CXCL1) and is highly homologous to CXCR1. It is highly expressed on neutrophils and is also found on monocytes, mast cells, and certain endothelial cells. Its main function is to serve as a master regulator of neutrophil chemotaxis, activation, and survival, driving their migration to inflammatory sites. Beyond its crucial role in acute inflammation and host defense, CXCR2 is also implicated in chronic inflammatory diseases (e.g., COPD, psoriasis), tumor progression (by promoting angiogenesis and immunosuppression), and tissue repair, making it a significant therapeutic target.

Screeningbio’s HEK293/Human CXCR2 cell line overexpress CXCR2 and is designed to detect inhibition in intracellular cAMP levels in response to agonist stimulation of the receptor. Cisbio HTRF cAMP kit can be used to detect the signal.

Product Specifications

Target Type	GPCR
Species	Human
HGNC Symbol	CXCR2
Accession Number	NM_001557
Parental Line	HEK293
Lot#	See Vial
Storage	Liquid Nitrogen

Data

HEK293/Human CXCR2 Agonist Assay. HEK293/Human CXCR2 cells were stimulated with 2.5 μM Forskolin and treated with reference agonist. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software. — **HEK293/Human CXCR2 Agonist Assay.** HEK293/Human CXCR2 cells were stimulated with 2.5 μM Forskolin and treated with reference agonist. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software.

Target Background

C-X-C motif chemokine receptor 2 (CXCR2) is a G protein-coupled receptor that primarily binds CXC family chemokines (such as IL-8/CXCL8 and GROα/CXCL1) and is highly homologous to CXCR1. It is highly expressed on neutrophils and is also found on monocytes, mast cells, and certain endothelial cells. Its main function is to serve as a master regulator of neutrophil chemotaxis, activation, and survival, driving their migration to inflammatory sites.

Beyond its crucial role in acute inflammation and host defense, CXCR2 is also implicated in chronic inflammatory diseases (e.g., COPD, psoriasis), tumor progression (by promoting angiogenesis and immunosuppression), and tissue repair, making it a significant therapeutic target.