HEK293 Human CXCR1 Stable Cell

HEK293/Human CXCR1 Stable Cell

Item	Cat#	Price
Stable Cell Line	SNB-G-0062A	$19,800
Compound Testing Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

C-X-C motif chemokine receptor 1 (CXCR1), also known as IL-8RA, is a G protein-coupled receptor that primarily binds interleukin-8 (IL-8/CXCL8). It is highly expressed on neutrophils and is also present on other immune cells such as monocytes and mast cells. Its main function is to mediate the directed chemotaxis and activation of neutrophils to sites of infection or injury, primarily by inducing actin polymerization and cell degranulation. It plays a central role in acute inflammatory responses, host defense against bacterial infections, and the pathology of certain inflammatory diseases (such as chronic obstructive pulmonary disease and rheumatoid arthritis). Consequently, CXCR1 is considered a key therapeutic target for modulating neutrophil-driven inflammation.

Screeningbio’s HEK293/Human CXCR1 cell line overexpress CXCR1 and is designed to detect inhibition in intracellular cAMP levels in response to agonist stimulation of the receptor. Cisbio HTRF cAMP kit can be used to detect the signal.

Product Specifications

Target Type	GPCR
Species	Human
HGNC Symbol	CXCR1
Accession Number	NM_000634
Parental Line	HEK293
Lot#	See Vial
Storage	Liquid Nitrogen

Data

HEK293/Human CXCR1 Agonist Assay. HEK293/Human CXCR1 cells were stimulated with 2.5 μM Forskolin and treated with reference agonist. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software. — **HEK293/Human CXCR1 Agonist Assay.** HEK293/Human CXCR1 cells were stimulated with 2.5 μM Forskolin and treated with reference agonist. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software.

Target Background

C-X-C motif chemokine receptor 1 (CXCR1), also known as IL-8RA, is a G protein-coupled receptor that primarily binds interleukin-8 (IL-8/CXCL8). It is highly expressed on neutrophils and is also present on other immune cells such as monocytes and mast cells. Its main function is to mediate the directed chemotaxis and activation of neutrophils to sites of infection or injury, primarily by inducing actin polymerization and cell degranulation.

It plays a central role in acute inflammatory responses, host defense against bacterial infections, and the pathology of certain inflammatory diseases (such as chronic obstructive pulmonary disease and rheumatoid arthritis). Consequently, CXCR1 is considered a key therapeutic target for modulating neutrophil-driven inflammation.