HEK293 Human CCK1 Stable Cell

HEK293/Human CCK1 Stable Cell

Item	Cat#	Price
Stable Cell Line	SNB-G-0075A	$19,800
Compound Testing Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

The Cholecystokinin A receptor (CCK1 or CCK-A) is a G protein-coupled receptor primarily responsive to the hormone cholecystokinin. It is predominantly distributed in the gastrointestinal system (e.g., gallbladder, pancreas, sphincter of Oddi, gastric and intestinal smooth muscle) and specific regions of the central nervous system (e.g., nucleus tractus solitarius). Its main function is to mediate the physiological actions of cholecystokinin, including stimulating gallbladder contraction and bile release, promoting pancreatic enzyme secretion, regulating gastric emptying and intestinal motility, and participating in the central regulation of behaviors such as satiety and anxiety. Consequently, CCK1 is a significant therapeutic target for modulating digestive functions and treating related disorders such as functional gastrointestinal disorders and obesity.

Screeningbio’s HEK293/Human CCK1 cell line overexpress CCK1 receptor and is designed to detect increases in intracellular IP-1 levels in response to agonist stimulation of the receptor. Cisbio HTRF IP-1 kit can be used to detect the signal.

Product Specifications

Target Type	GPCR
Species	Human
HGNC Symbol	CCK1
Accession Number	NM_000730
Parental Line	HEK293
Lot#	See Vial
Storage	Liquid Nitrogen

Data

HEK293/Human CCK1 Agonist Assay. HEK293/Human CCK1 cells were treated with the reference agonist. The assay was run based on Revvity IP-one HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 /IC50 values were determined, using GraphPad Prism software. — **HEK293/Human CCK1 Agonist Assay.** HEK293/Human CCK1 cells were treated with the reference agonist. The assay was run based on Revvity IP-one HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 /IC50 values were determined, using GraphPad Prism software.

Target Background

The Cholecystokinin A receptor (CCK1 or CCK-A) is a G protein-coupled receptor primarily responsive to the hormone cholecystokinin. It is predominantly distributed in the gastrointestinal system (e.g., gallbladder, pancreas, sphincter of Oddi, gastric and intestinal smooth muscle) and specific regions of the central nervous system (e.g., nucleus tractus solitarius).

Its main function is to mediate the physiological actions of cholecystokinin, including stimulating gallbladder contraction and bile release, promoting pancreatic enzyme secretion, regulating gastric emptying and intestinal motility, and participating in the central regulation of behaviors such as satiety and anxiety. Consequently, CCK1 is a significant therapeutic target for modulating digestive functions and treating related disorders such as functional gastrointestinal disorders and obesity.