HEK293 Cyno APJ Stable Cell

HEK293/Cyno APJ Stable Cell

Item	Cat#	Price
Stable Cell Line	SNB-G-0033L	$19,800
Compound Testing Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

The APJ receptor (Apelin receptor, also known as APLNR) belongs to the Class A family of G protein-coupled receptors (GPCRs), which is the most extensively studied GPCR family and has the highest concentration of drug targets. The APJ receptor and its ligands are involved in numerous physiological functions and pathophysiological processes, making it a pivotal therapeutic target for future treatments of conditions such as ischemic heart disease, hypertension, and heart failure.

Screeningbio’s HEK293/Cyno APJ cell line overexpress APJ and is designed to detect increases in intracellular cAMP levels in response to agonist stimulation of the receptor. Cisbio HTRF cAMP kit can be used to detect the signal.

Product Specifications

Target Type	GPCR
Species	Macaca fascicularis (Cyno)
HGNC Symbol	APLNR
Accession Number	XM_005577873.4
Parental Line	HEK293
Lot#	See Vial
Storage	Liquid Nitrogen

Data

HEK293/Cyno APJ Agonist Assay. HEK293/Cyno APJ cells were stimulated with 5 μM Forskolin and treated with reference agonist Apelin-13. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software. — **HEK293/Cyno APJ Agonist Assay.** HEK293/Cyno APJ cells were stimulated with 5 μM Forskolin and treated with reference agonist Apelin-13. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software.

Target Background

The APJ receptor (Apelin receptor, also known as APLNR) belongs to the Class A family of G protein-coupled receptors (GPCRs), which is the most extensively studied GPCR family and has the highest concentration of drug targets.

The APJ receptor and its ligands are involved in numerous physiological functions and pathophysiological processes, making it a pivotal therapeutic target for future treatments of conditions such as ischemic heart disease, hypertension, and heart failure.