Product Description

The Orexin Receptor 1 (OXR1), also known as HCRTR1, is a G protein-coupled receptor (GPCR) primarily expressed in the hypothalamic region. By binding to its endogenous ligand orexin-A, it activates intracellular signaling cascades mediated by Gq proteins. Its core functions include regulating energy metabolism balance, stress responses, and the sleep-wake cycle. Dysregulation of OX1R is associated with insomnia, narcolepsy, and obesity.

ScreeningBio’s CHO-K1/Rat OX1 β-arrestin cell line is an ideal tool for studying GPCR/β-arrestin interactions. In this system, the GPCR C-terminus is fused to a smallBiT tag, and the β2-arrestin N-terminus is fused to a largeBiT tag. Upon receptor activation, GPCR/β-arrestin interaction brings the two fragments together to reconstitute an active NanoLuc enzyme, which can be quantified using the NanoBiT substrate. This cell line is designed to evaluate a compound’s ability to activate the β-arrestin signaling pathway.