Product Description

 M1 (Cholinergic receptor muscarinic 1, CHRM1) is a member of the G protein-coupled receptor family, primarily distributed in the central nervous system, retina, and urinary system. By binding to acetylcholine, M1 activates G proteins and downstream signaling pathways, thus mediating diverse cellular responses. The biological significance of M1 is very broad—it is involved in regulating vagus nerve-induced bronchoconstriction, acid secretion in the gastrointestinal tract, and cognitive functions, among others. Furthermore, its dysfunction is associated with a variety of diseases.

ScreeningBio’s CHO-K1/Rat M1 β-arrestin cell line is an ideal tool for studying GPCR/β-arrestin interactions. In this system, the GPCR C-terminus is fused to a smallBiT tag, and the β2-arrestin N-terminus is fused to a largeBiT tag. Upon receptor activation, GPCR/β-arrestin interaction brings the two fragments together to reconstitute an active NanoLuc enzyme, which can be quantified using the NanoBiT substrate. This cell line is designed to evaluate a compound’s ability to activate the β-arrestin signaling pathway.