CHO-K1 Rat CXCR4 Stable Cell

CHO-K1/Rat CXCR4 Stable Cell

Item	Cat#	Price
Stable Cell Line	SNB-G-0065C	$19,800
Compound Testing Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

C-X-C motif chemokine receptor 4 (CXCR4) is a ubiquitously expressed G protein-coupled receptor whose sole known natural ligand is stromal cell-derived factor-1 (SDF-1/CXCL12). It is expressed on a wide variety of immune cells, hematopoietic stem cells, endothelial cells, and cancer cells. The core function of CXCR4 is to mediate directed cell migration and homing, playing a crucial role in embryonic development, bone marrow homing of hematopoietic stem cells, and immune cell trafficking. Furthermore, CXCR4 serves as one of the primary co-receptors for HIV viral entry and plays a key role in tumor metastasis, angiogenesis, and inflammation, making it a critical therapeutic target for AIDS, cancer, and autoimmune diseases.

Screeningbio’s CHO-K1/Rat CXCR4 cell line overexpress CXCR4 and is designed to detect inhibition in intracellular cAMP levels in response to agonist stimulation of the receptor. Cisbio HTRF cAMP kit can be used to detect the signal.

Product Specifications

Target Type	GPCR
Species	Rat
HGNC Symbol	CXCR4
Accession Number	NM_022205
Parental Line	CHO-K1
Lot#	See Vial
Storage	Liquid Nitrogen

Data

CHO-K1/Rat CXCR4 Agonist Assay. CHO-K1/Rat CXCR4 cells were stimulated with 2.5 μM Forskolin and treated with reference agonist. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software. — **CHO-K1/Rat CXCR4 Agonist Assay.** CHO-K1/Rat CXCR4 cells were stimulated with 2.5 μM Forskolin and treated with reference agonist. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software.

Target Background

C-X-C motif chemokine receptor 4 (CXCR4) is a ubiquitously expressed G protein-coupled receptor whose sole known natural ligand is stromal cell-derived factor-1 (SDF-1/CXCL12). It is expressed on a wide variety of immune cells, hematopoietic stem cells, endothelial cells, and cancer cells.

The core function of CXCR4 is to mediate directed cell migration and homing, playing a crucial role in embryonic development, bone marrow homing of hematopoietic stem cells, and immune cell trafficking. Furthermore, CXCR4 serves as one of the primary co-receptors for HIV viral entry and plays a key role in tumor metastasis, angiogenesis, and inflammation, making it a critical therapeutic target for AIDS, cancer, and autoimmune diseases.