Product Description

Chemokine (C-C motif) receptor 7 (CCR7) is a crucial G protein-coupled receptor whose ligands are CCL19 and CCL21. It is primarily expressed on naïve T cells, central memory T cells, and mature dendritic cells. The core function of CCR7 is to guide the migration of these cells from peripheral tissues or the bloodstream into the T-cell zones of secondary lymphoid organs (such as lymph nodes), which is a critical step for immune response initiation and immune surveillance. By mediating the homing of dendritic cells to lymph nodes for antigen presentation and guiding T cells to be activated there, CCR7 plays an indispensable role in initiating adaptive immunity, the development of lymphoid tissues, and lymphocyte homing.

ScreeningBio’s HEK293T/Mouse CCR7 β-arrestin cell line is an ideal tool for studying GPCR/β-arrestin interactions. In this system, the GPCR C-terminus is fused to a smallBiT tag, and the β2-arrestin N-terminus is fused to a largeBiT tag. Upon receptor activation, GPCR/β-arrestin interaction brings the two fragments together to reconstitute an active NanoLuc enzyme, which can be quantified using the NanoBiT substrate. This cell line is designed to evaluate a compound’s ability to activate the β-arrestin signaling pathway.