CHO-K1 Human Y2 Stable Cell

CHO-K1/Human Y2 Stable Cell

Item	Cat#	Price
Stable Cell Line	SNB-G-0167A	$19,800
Compound Testing Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

The neuropeptide Y receptor Y2 is a G protein-coupled receptor that primarily functions as a presynaptic autoreceptor. It is widely distributed in the central nervous system (e.g., hippocampus, hypothalamus) and peripheral tissues (e.g., enteric neurons and blood vessels). Its core function is to exert potent negative feedback inhibition on the release of neuropeptide Y and other neurotransmitters (such as glutamate) via the Gi/o protein signaling pathway, thereby finely regulating diverse physiological processes including anxiety, feeding behavior, memory consolidation, and gastrointestinal motility. It is a potential therapeutic target for obesity, anxiety disorders, and irritable bowel syndrome.

Screeningbio’s CHO-K1/Human Y2 cell line overexpress NPY2R and is designed to detect inhibition in intracellular cAMP levels in response to agonist stimulation of the receptor. Cisbio HTRF cAMP kit can be used to detect the signal.

Product Specifications

Target Type	GPCR
Species	Human
HGNC Symbol	NPY2R
Accession Number	SNB-G-0167A
Parental Line	CHO-K1
Lot#	See Vial
Storage	Liquid Nitrogen

Data

CHO-K1/Human Y2 Agonist Assay. CHO-K1/Human Y2 cells were stimulated with 2.5 μM Forskolin and treated with reference agonist. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software. — **CHO-K1/Human Y2 Agonist Assay.** CHO-K1/Human Y2 cells were stimulated with 2.5 μM Forskolin and treated with reference agonist. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software.

Target Background

The neuropeptide Y receptor Y2 is a G protein-coupled receptor that primarily functions as a presynaptic autoreceptor.

It is widely distributed in the central nervous system (e.g., hippocampus, hypothalamus) and peripheral tissues (e.g., enteric neurons and blood vessels). Its core function is to exert potent negative feedback inhibition on the release of neuropeptide Y and other neurotransmitters (such as glutamate) via the Gi/o protein signaling pathway, thereby finely regulating diverse physiological processes including anxiety, feeding behavior, memory consolidation, and gastrointestinal motility.

It is a potential therapeutic target for obesity, anxiety disorders, and irritable bowel syndrome.