CHO-K1 Human PKR1 Stable Cell

CHO-K1/Human PKR1 Stable Cell

Item	Cat#	Price
Stable Cell Line	SNB-G-0191A	$19,800
Compound Testing Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

PKR1 (Prokineticin receptor 1) is a Gq protein-coupled receptor encoded by the PROKR1 gene, belonging to the prokineticin receptor family. It is primarily distributed in peripheral tissues, including the cardiovascular system, gastrointestinal tract, reproductive organs, adipose tissue, and certain immune cells. Its core function involves binding to ligands prokineticin 1 (PK1) and prokineticin 2 (PK2) to activate phospholipase C and calcium mobilization, mediating cell survival, proliferation, and angiogenesis. It plays a crucial role in cardioprotection (anti-apoptosis, promoting angiogenesis), metabolic regulation (improving insulin resistance), inflammatory responses, and pain perception.

Screeningbio’s CHO-K1/Human PKR1 cell line overexpress PROKR1 receptor and is designed to detect increases in intracellular IP-1 levels in response to agonist stimulation of the receptor. Cisbio HTRF IP-1 kit can be used to detect the signal.

Product Specifications

Target Type	GPCR
Species	Human
HGNC Symbol	PROKR1
Accession Number	NM_138964 (Hs)
Parental Line	CHO-K1
Lot#	See Vial
Storage	Liquid Nitrogen

Data

CHO-K1/Human PKR1 Agonist Assay. CHO-K1/Human PKR1 cells were treated with the reference agonist. The assay was run based on Revvity IP-one HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 /IC50 values were determined, using GraphPad Prism software. — **CHO-K1/Human PKR1 Agonist Assay.** CHO-K1/Human PKR1 cells were treated with the reference agonist. The assay was run based on Revvity IP-one HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 /IC50 values were determined, using GraphPad Prism software.

Target Background

PKR1 (Prokineticin receptor 1) is a Gq protein-coupled receptor encoded by the PROKR1 gene, belonging to the prokineticin receptor family. It is primarily distributed in peripheral tissues, including the cardiovascular system, gastrointestinal tract, reproductive organs, adipose tissue, and certain immune cells.

Its core function involves binding to ligands prokineticin 1 (PK1) and prokineticin 2 (PK2) to activate phospholipase C and calcium mobilization, mediating cell survival, proliferation, and angiogenesis. It plays a crucial role in cardioprotection (anti-apoptosis, promoting angiogenesis), metabolic regulation (improving insulin resistance), inflammatory responses, and pain perception.