Product Description

The OT receptor (oxytocin receptor) is a Gq protein-coupled receptor encoded by the OXTR gene, primarily distributed in uterine smooth muscle (especially during labor), mammary myoepithelial cells, as well as the central nervous system (e.g., hypothalamus, amygdala, hippocampus) and peripheral tissues such as the kidneys. Its core function, upon activation by oxytocin, involves mediating the phospholipase C signaling pathway via Gq protein to elevate intracellular calcium, thereby inducing uterine contractions to facilitate labor, triggering milk ejection, and modulating social behavior, emotional bonding, trust, and anxiolytic effects in the central nervous system. This receptor serves as the direct target of uterotonic drugs used in obstetric labor induction and is also associated with neuropsychiatric conditions including autism and postpartum depression.

ScreeningBio’s CHO-K1/Human OT β-arrestin cell line is an ideal tool for studying GPCR/β-arrestin interactions. In this system, the GPCR C-terminus is fused to a smallBiT tag, and the β2-arrestin N-terminus is fused to a largeBiT tag. Upon receptor activation, GPCR/β-arrestin interaction brings the two fragments together to reconstitute an active NanoLuc enzyme, which can be quantified using the NanoBiT substrate. This cell line is designed to evaluate a compound’s ability to activate the β-arrestin signaling pathway.