Product Description

Hydroxycarboxylic acid receptor 1 (HCA1), formerly known as GPR81, is a G protein-coupled receptor that specifically senses lactate signaling. It is primarily distributed in adipose tissue (white and brown fat), skeletal muscle, the liver, and the central nervous system. Its core function is to serve as a critical metabolic “braking” signal: in adipose tissue, activation by lactate via the Gi protein pathway potently inhibits lipolysis, reducing fatty acid release; it also participates in regulating insulin sensitivity, hepatic glucose output, and neurometabolism. Thus, HCA1 is a vital hub connecting energy metabolism and inter-tissue communication, with its dysfunction closely associated with metabolic disorders such as obesity, insulin resistance, and non-alcoholic fatty liver disease.

Screeningbio’s CHO-K1/Human HCA1 cell line inducibly express HCAR1 and is designed to detect inhibition in intracellular cAMP levels in response to agonist stimulation of the receptor. Cisbio HTRF cAMP kit can be used to detect the signal.