Product Description
GSK3β (Glycogen synthase kinase 3 beta), also known as GSK3B, is a member of the serine/threonine protein kinase family that plays a pivotal role in glycogen metabolism, Wnt/β-catenin signaling, cell proliferation, neuronal development, apoptosis, and circadian rhythm regulation. GSK3β is constitutively active under resting conditions and is inhibited by phosphorylation at Ser9 (by AKT, PKA, PKC, or integrin-linked kinase) in response to insulin, growth factors, and Wnt ligands (through LRP5/6 co-receptors), leading to downstream regulation of numerous substrates including glycogen synthase, β-catenin, tau protein, cyclin D1, c-Myc, and NF-κB. Beyond its role in normal cellular homeostasis, energy balance, and neurogenesis, dysregulation of GSK3β signaling—either through hyperactivation or impaired inhibition—is closely associated with type 2 diabetes mellitus, Alzheimer's disease (tau hyperphosphorylation and amyloid-beta toxicity), Parkinson's disease, bipolar disorder, major depression, and various cancers (including colorectal, pancreatic, and glioblastoma), making it a highly validated target for metabolic disorders, neurodegenerative diseases, psychiatric conditions, and drug discovery.
Screeningbio’s GSK3β Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying GSK3β kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: GSK3β catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the GSK3β kinase.
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