Product Description
CK1ε (Casein kinase 1 epsilon), also known as CSNK1E, is a member of the serine/threonine kinase family that plays a pivotal role in regulating circadian rhythm, Wnt/β-catenin signaling, cell cycle progression, and membrane trafficking. CK1ε phosphorylates key substrates such as PERIOD proteins (critical components of the circadian clock), β-catenin, and Dishevelled, thereby controlling protein stability, subcellular localization, and transcriptional activity. Beyond its essential physiological functions in maintaining circadian rhythms and cellular homeostasis, dysregulation of CK1ε signaling—including overexpression, gain-of-function mutations, and aberrant pathway modulation—is closely associated with circadian rhythm disorders, neurodegenerative diseases (such as Alzheimer’s disease), and various cancers (including breast cancer, colorectal cancer, and leukemia), making it a highly validated target for therapeutic intervention in chronobiology, neurology, and oncology.
Screeningbio’s CK1ε Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying CK1ε kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: CK1ε catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the CK1ε kinase.
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