Product Description
CK1γ1 (Casein kinase 1 gamma 1), also known as CSNK1G1, is a member of the serine/threonine kinase family that plays a pivotal role in regulating cell signaling pathways, including Wnt/β-catenin signaling, membrane trafficking, and cytoskeletal dynamics. CK1γ1 is distinguished from other CK1 family members by its membrane association via palmitoylation and its ability to phosphorylate key substrates such as Dishevelled, LRP5/6, and various membrane-associated proteins, thereby modulating signal transduction and cellular polarity. Beyond its essential physiological functions in development and cellular organization, dysregulation of CK1γ1 signaling—including aberrant expression and altered kinase activity—is closely associated with various cancers (such as breast cancer, colorectal cancer, and glioblastoma), neurological disorders, and inflammatory diseases, making it a highly validated target for cancer therapy and drug discovery.
Screeningbio’s CK1γ1 Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying CK1γ1 kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: CK1γ1 catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the CK1γ1 kinase.
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