Product Description
CDK5/p35 is a serine/threonine kinase complex composed of the catalytic subunit CDK5 (cyclin-dependent kinase 5) and its regulatory activator p35, which plays a pivotal role in neuronal development, synaptic plasticity, and cytoskeletal organization. Unlike other CDK family members, CDK5 is primarily activated by the non-cyclin proteins p35 or p39, and the CDK5/p35 complex is essential for proper neuronal migration, axonal guidance, and synaptic function. Beyond its essential physiological functions in the central nervous system, dysregulation of CDK5/p35 signaling—including altered expression, aberrant proteolytic conversion of p35 to p25, and hyperactivation—is closely associated with neurodegenerative diseases (such as Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis), neuroinflammation, and cancer progression, making it a highly validated target for therapeutic intervention in neurology and oncology.
Screeningbio’s CDK5/p35 Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying CDK5/p35 kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: CDK5/p35 catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the CDK5/p35 kinase.
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