Product Description
CDK5/p25 is a serine/threonine kinase complex composed of the catalytic subunit CDK5 (cyclin-dependent kinase 5) and its atypical regulatory activator p25, a proteolytic cleavage product of p35, which plays a pivotal role in neuronal development, synaptic plasticity, and cytoskeletal dynamics. Unlike other CDK family members, CDK5 is primarily activated by p35 or p39 rather than cyclins, and the conversion of p35 to p25 results in prolonged and aberrant CDK5 activation. Beyond its essential physiological functions in neuronal migration, axonal guidance, and synaptic function, dysregulation of CDK5/p25 signaling—particularly through pathological p25 accumulation—is closely associated with neurodegenerative diseases (including Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis), as well as neuroinflammation, diabetes, and cancer progression. These features have established CDK5/p25 as a highly validated target for therapeutic intervention in neurology, oncology, and metabolic disorders.
Screeningbio’s CDK5/p25 Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying CDK5/p25 kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: CDK5/p25 catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the CDK5/p25 kinase.
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