Product Description
AMPK (A2/B1/G1) is a heterotrimeric complex composed of the catalytic subunit α2 (PRKAA2), the scaffold subunit β1 (PRKAB1), and the regulatory subunit γ1 (PRKAG1), and belongs to the AMP‑activated protein kinase family, which plays a pivotal role in cellular energy homeostasis and metabolic stress sensing. The A2/B1/G1 isoform is activated by changes in the AMP/ATP ratio, as well as by upstream kinases such as LKB1 and CaMKK2, leading to phosphorylation of downstream targets that regulate glucose and lipid metabolism, autophagy, and mitochondrial biogenesis. Beyond its essential role in energy balance, dysregulation of AMPK (A2/B1/G1) signaling is closely associated with metabolic disorders (including type 2 diabetes and obesity), cardiovascular diseases, inflammation, and cancer progression, making it a highly validated target for drug discovery in metabolic and oncology indications.
Screeningbio’s AMPK(A2/B1/G1) Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying AMPK(A2/B1/G1) kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: AMPK(A2/B1/G1) catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the AMPK(A2/B1/G1) kinase.
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