HEK293 LXRα Luciferase Reporter Cell Line

HEK293/LXRα Luciferase Reporter Cell Line

Item	Cat#	Price
Stable Cell Line	SNB-NR-0005	$19,800
Compound Testing Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

LXRα (Liver X Receptor alpha) is a ligand-activated nuclear receptor and a member of the nuclear receptor superfamily that functions as a cholesterol sensor. It is primarily distributed in highly metabolically active tissues such as the liver, adipose tissue, macrophages, intestine, and kidneys. The main functions of LXRα include regulating cholesterol and lipid metabolism and transport, promoting the conversion of cholesterol into bile acids, suppressing inflammatory responses, and playing a key role in maintaining systemic lipid homeostasis. It is also closely associated with metabolic diseases such as atherosclerosis and fatty liver disease.

Screeningbio’s HEK293/LXRα Luciferase Reporter cell line contains human LXRα gene and a luciferase gene under the control of live X response elements. The cell line is designed to detect the response to the stimulation of LXRα ligands. Promega One-Glo kit can be used to detect the signal.

Product Specifications

Target Type	Nuclear Receptor
Species	Human
HGNC Symbol	NR1H3
Accession Number	NM_001130102 (Hs)
Parental Line	HEK293
Lot#	See Vial
Storage	Liquid Nitrogen

Data

HEK293/LXRα Luciferase Reporter Agonist Assay. HEK293/LXRα Luciferase Reporter cells were treated with agonist. The assay was run based on Promega ONE-GLOTM Luciferase Assay System. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software. — **HEK293/LXRα Luciferase Reporter Agonist Assay.** HEK293/LXRα Luciferase Reporter cells were treated with agonist. The assay was run based on Promega ONE-GLO^TM Luciferase Assay System. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software.

HEK293/LXRα Luciferase Reporter Antagonist Assay. HEK293/LXRα Luciferase Reporter cells were treated with antagonist. The assay was run based on Promega ONE-GLOTM Luciferase Assay System. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software. — **HEK293/LXRα Luciferase Reporter Antagonist Assay.** HEK293/LXRα Luciferase Reporter cells were treated with antagonist. The assay was run based on Promega ONE-GLO^TM Luciferase Assay System. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software.

Target Background

LXRα (Liver X Receptor alpha) is a ligand-activated nuclear receptor and a member of the nuclear receptor superfamily that functions as a cholesterol sensor. It is primarily distributed in highly metabolically active tissues such as the liver, adipose tissue, macrophages, intestine, and kidneys.

The main functions of LXRα include regulating cholesterol and lipid metabolism and transport, promoting the conversion of cholesterol into bile acids, suppressing inflammatory responses, and playing a key role in maintaining systemic lipid homeostasis. It is also closely associated with metabolic diseases such as atherosclerosis and fatty liver disease.