Product Description

CRF1 receptor (CRHR1), a G protein-coupled receptor, is the primary receptor for corticotropin-releasing hormone. It is widely distributed in brain regions involved in stress and emotional regulation, such as the amygdala, hypothalamus, and cerebral cortex, and also expressed in the anterior pituitary and immune system. As the "master switch" of the body's stress response, its activation initiates the hypothalamic-pituitary-adrenal axis, leading to cortisol release. It directly regulates behaviors like anxiety, depression, learning, and memory. Consequently, CRF1 receptor antagonists represent a major research direction for developing novel therapies for depression, anxiety, and other stress-related psychiatric disorders.

ScreeningBio’s HEK293T/Rat CRF1 β-Arrestin cell line is an ideal tool for studying GPCR/β-arrestin interactions. In this system, the GPCR C-terminus is fused to a smallBiT tag, and the β2-arrestin N-terminus is fused to a largeBiT tag. Upon receptor activation, GPCR/β-arrestin interaction brings the two fragments together to reconstitute an active NanoLuc enzyme, which can be quantified using the NanoBiT substrate. This cell line is designed to evaluate a compound’s ability to activate the β-arrestin signaling pathway.