HEK293T/Rat B2 β-Arrestin Stable Cell
Item | Cat# | Price |
Stable Cell Line | SNB-A-0039C | Inquiry |
Compound Testing Services | CT-001 | $1,850 per 384w plate (Up To 16 cpds Dose) |
Product Description
The bradykinin B2 receptor is a widely and constitutively expressed G protein-coupled receptor that serves as the primary mediator for the kinin system. It is ubiquitously present on vascular endothelial cells, smooth muscle cells, and neurons. Its key functions include mediating acute vasodilation (leading to hypotension), increasing vascular permeability (causing edema), and eliciting pain and cough reflexes. It also plays a critical role in the pathophysiology of shock.
ScreeningBio’s HEK293T/Rat B2 β-arrestin cell line is an ideal tool for studying GPCR/β-arrestin interactions. In this system, the GPCR C-terminus is fused to a smallBiT tag, and the β2-arrestin N-terminus is fused to a largeBiT tag. Upon receptor activation, GPCR/β-arrestin interaction brings the two fragments together to reconstitute an active NanoLuc enzyme, which can be quantified using the NanoBiT substrate. This cell line is designed to evaluate a compound’s ability to activate the β-arrestin signaling pathway.
Product Specifications
Target Type | GPCR |
Species | Rat |
HGNC Symbol | B2 receptor |
Accession Number | NM_173100 (Rn) |
Parental Line | HEK293T |
Lot# | See Vial |
Storage | Liquid Nitrogen |
Data
Target Background
The bradykinin B2 receptor is a widely and constitutively expressed G protein-coupled receptor that serves as the primary mediator for the kinin system. It is ubiquitously present on vascular endothelial cells, smooth muscle cells, and neurons. Its key functions include mediating acute vasodilation (leading to hypotension), increasing vascular permeability (causing edema), and eliciting pain and cough reflexes. It also plays a critical role in the pathophysiology of shock.
ScreeningBio’s HEK293T/Rat B2 β-arrestin cell line is an ideal tool for studying GPCR/β-arrestin interactions. In this system, the GPCR C-terminus is fused to a smallBiT tag, and the β2-arrestin N-terminus is fused to a largeBiT tag. Upon receptor activation, GPCR/β-arrestin interaction brings the two fragments together to reconstitute an active NanoLuc enzyme, which can be quantified using the NanoBiT substrate. This cell line is designed to evaluate a compound’s ability to activate the β-arrestin signaling pathway.