HEK293 Human D5 Stable Cell

HEK293/Human D5 Stable Cell

Item	Cat#	Price
Stable Cell Line	SNB-G-0086A	$19,800
Compound Testing Services	CT-001	$1,850 per 384w plate *(Up To 16 cpds Dose)*

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Product Description

The dopamine D5 receptor is a key member of the D1-like receptor family. Structurally, it is highly homologous to the D1 receptor but exhibits a higher basal affinity for dopamine. Its distribution in the brain is more restricted than that of D1, being highly enriched in the hippocampus, hypothalamus, and prefrontal cortex—regions critically involved in cognition and emotion. Its primary function is to activate the Gs/cAMP signaling pathway, thereby participating in the regulation of learning, memory, emotional responses, and neuronal plasticity. Its expression in the kidneys and blood vessels also suggests a role in blood pressure regulation. Consequently, dysfunction of the D5 receptor has been implicated in the cognitive deficits of schizophrenia, the pathological processes of Alzheimer's disease, and the pathogenesis of hypertension, making it a potential therapeutic target.

Screeningbio’s HEK293/Human D5 cell line overexpress DRD5 and is designed to detect increases in intracellular cAMP levels in response to agonist stimulation of the receptor. Cisbio HTRF cAMP kit can be used to detect the signal.

Product Specifications

Target Type	GPCR
Species	Human
HGNC Symbol	DRD5
Accession Number	NM_000798
Parental Line	HEK293
Lot#	See Vial
Storage	Liquid Nitrogen

Data

HEK293/Human D5 Agonist Assay. HEK293/Human D5 cells were treated with the reference agonist. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software. — **HEK293/Human D5 Agonist Assay.** HEK293/Human D5 cells were treated with the reference agonist. The assay was run based on Revvity cAMP HTRF protocol. Non-linear regression was used to plot activity changes vs. [Compound, M], and EC50 values were determined, using GraphPad Prism software.

Target Background

The dopamine D5 receptor is a key member of the D1-like receptor family. Structurally, it is highly homologous to the D1 receptor but exhibits a higher basal affinity for dopamine.

Its distribution in the brain is more restricted than that of D1, being highly enriched in the hippocampus, hypothalamus, and prefrontal cortex—regions critically involved in cognition and emotion. Its primary function is to activate the Gs/cAMP signaling pathway, thereby participating in the regulation of learning, memory, emotional responses, and neuronal plasticity.

Its expression in the kidneys and blood vessels also suggests a role in blood pressure regulation. Consequently, dysfunction of the D5 receptor has been implicated in the cognitive deficits of schizophrenia, the pathological processes of Alzheimer's disease, and the pathogenesis of hypertension, making it a potential therapeutic target.