Product Description

The complement C5a receptor 1 is a G protein-coupled receptor primarily expressed on the surface of myeloid immune cells such as neutrophils, monocytes, macrophages, and mast cells. Its core function is to efficiently recognize the complement activation product C5a, driving acute inflammatory responses and host defense by mediating robust chemotaxis, degranulation, and inflammatory cytokine release from immune cells. Excessive activation of this receptor is closely associated with inflammatory diseases including sepsis, acute lung injury, and rheumatoid arthritis.

ScreeningBio’s Mouse C5A1 β-arrestin cell line is an ideal tool for studying GPCR/β-arrestin interactions. In this system, the GPCR C-terminus is fused to a smallBiT tag, and the β2-arrestin N-terminus is fused to a largeBiT tag. Upon receptor activation, GPCR/β-arrestin interaction brings the two fragments together to reconstitute an active NanoLuc enzyme, which can be quantified using the NanoBiT substrate. This cell line is designed to evaluate a compound’s ability to activate the β-arrestin signaling pathway.