Product Description

The Orexin Receptor 2 (OXR2), also known as HCRTR2, is a G protein-coupled receptor primarily expressed in regions such as the cerebral cortex, hippocampus, and hypothalamus. OXR2 plays important physiological roles by regulating the sleep-wake cycle, mood, learning and memory, feeding behavior, reward/addiction, and energy balance. Dysfunction of OXR2 is closely associated with sleep disorders and is involved in the pathophysiology of conditions such as insomnia, hypersomnia, depression, and narcolepsy.

ScreeningBio’s CHO-K1/Human OX2 β-arrestin cell line is an ideal tool for studying GPCR/β-arrestin interactions. In this system, the GPCR C-terminus is fused to a smallBiT tag, and the β2-arrestin N-terminus is fused to a largeBiT tag. Upon receptor activation, GPCR/β-arrestin interaction brings the two fragments together to reconstitute an active NanoLuc enzyme, which can be quantified using the NanoBiT substrate. This cell line is designed to evaluate a compound’s ability to activate the β-arrestin signaling pathway.