Product Description

The complement C5a receptor 2 (C5AR2, also known as C5L2) is another G protein-coupled receptor for C5a. It shares a similar distribution pattern with C5AR1 (e.g., neutrophils, monocytes, adipocytes) but lacks typical chemotactic functions. It is primarily regarded as a "decoy/modulatory receptor," regulating C5AR1 signaling and ligand internalization by binding with high affinity to both C5a and its metabolite C5a des-Arg. It also influences energy metabolism, playing a complex role in the regulatory networks of inflammatory and metabolic diseases.

ScreeningBio’s human C5A2 β-arrestin cell line is an ideal tool for studying GPCR/β-arrestin interactions. In this system, the GPCR C-terminus is fused to a smallBiT tag, and the β2-arrestin N-terminus is fused to a largeBiT tag. Upon receptor activation, GPCR/β-arrestin interaction brings the two fragments together to reconstitute an active NanoLuc enzyme, which can be quantified using the NanoBiT substrate. This cell line is designed to evaluate a compound’s ability to activate the β-arrestin signaling pathway.