Product Description
MINK1 (Misshapen-like kinase 1), also known as MAP4K6 (mitogen-activated protein kinase kinase kinase kinase 6) or YSK2, is a member of the serine/threonine protein kinase family (STE20/GCK subfamily) that plays a pivotal role in cytoskeletal dynamics, cell migration, neurite outgrowth, and immune synapse formation, as well as in JNK signaling, apoptosis, and T-cell activation. MINK1 is activated by various stimuli including TCR engagement, oxidative stress, and cell adhesion signals, leading to downstream activation of the JNK and p38 MAPK pathways, as well as phosphorylation of substrates such as NCK-interacting kinase (NIK) and Par-6, thereby regulating actin reorganization and cell polarity. Beyond its role in normal neuronal development and immune function, dysregulation of MINK1 signaling—through loss-of-function mutations, aberrant expression, or altered kinase activity—is closely associated with multiple cancers (including breast cancer, colorectal cancer, and glioblastoma), neurodegenerative diseases, and immune disorders, making it a highly validated target for cancer therapy and drug discovery.
Screeningbio’s MINK1 Kinase Assay Kit provides a robust, sensitive, and high-throughput platform for quantifying MINK1 kinase activity and evaluating the potency of tyrosine kinase inhibitors (TKIs).
This kit utilizes the ADP-Glo Kinase Assay platform, a luminescent technology that measures the amount of ADP produced during the kinase reaction.
The assay is performed in two distinct steps:
Kinase Reaction: MINK1 catalyzes the transfer of a phosphate group from ATP to a specific substrate, resulting in the production of phosphorylated substrate and ADP.
ADP-Glo™ Reagent Addition: After the kinase reaction, the ADP-Glo™ Reagent is added to terminate the reaction and deplete any remaining unreacted ATP.
Kinase Detection: The Kinase Detection Reagent is added to simultaneously convert the produced ADP back into ATP, which is then used by luciferase to generate a luminescent signal.
The light intensity produced is directly proportional to the ADP concentration and, consequently, to the enzymatic activity of the MINK1 kinase.
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