离子通道 (Ion Channel)

 

Ion channels are pore-forming proteins that allow the flow of ions across membranes, either plasma membranes, or the membranes of intracellular organelles. Ion channels represent the second largest target for existing drugs after G protein-coupled receptors, the therapeutic areas including arrhythmia, hypertension, local anesthesia, pain, stroke, epilepsy, depression, bipolar disorder, cystic fibrosis, cardiac arrhythmias, COPD, autoimmune disorders, and diabetes. The advent of novel, faster screening techniques for compounds acting on ion channels suggests that these proteins represent promising targets for the development of additional, novel therapeutic agents for the near future.

 

Ion channels not only constitute a significant class of drug targets, they are also critical for evaluating drug safety including being part of the FDA required safety testing for any drug targeted against human disease.

 

ScreeNingBio provide functionally validated ion channel products for use in cell-based assays, and an hERG ion channel membrane preps for Fluorescence Polarization (FP) binding studies. These assays are suitable for the compounds profiling and high throughput screening.

 

Calcium Assays: Fluorescent dye-based assays to measure calcium mobilization in cell lines as a direct indication of Ion channel activation or inhibition.

Membrane potential Assays: Fluorescent dye-based assays to measure membrane potential changes associated with Ion channel activation or inhibition.

Thallium Assays: Fluorescent dye-based assays to measure thallium mobilization in cell lines as a direct indication of Ion channel activation or inhibition.

Fluorescence Polarization Assays: Purified hERG membrane preps for obtaining ligand binding affinities to identify potential hERG channel blockers by producing data that accurately correlates with patch-clamp electrophysiology studies.

 

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