Chemical - In vitro 3T3 NRU phototoxicity test method

The in vitro 3T3 Neutral Red Uptake (NRU) phototoxicity test is used to identify the phototoxic potential of a test chemical activated by exposure to light. The test evaluates photo-cytotoxicity by the relative reduction in viability of cells exposed to the test chemical in the presence versus absence of light. Chemicals identified as positive in this test may be phototoxic in vivo, following topical application or systemic application and distribution to the skin and/or eyes.